"外源酚酸对杨树幼苗根系生理和形态发育的影响.pdf:"

作者：杨阳，王华田，王延平，姜岳忠，王宗芹

单位：山东农业大学农业生态环境实验室，山东农业大学林学院，山东省淄博市鲁山林场，山东省林业科学研究院

刊物：林业科学

卷期：2010，46（11）

页码：73-80

索引格式：
杨阳, 王华田, 王延平, 姜岳忠, 王宗芹. 外源酚酸对杨树幼苗根系生理和形态发育的影响[J]. 林业科学, 2010, 46(11): 73 – 80

摘要：

    连作杨树人工林地力衰退与杨树根际酚酸物质的累积密切相关，深入研究杨树根系在酚酸环境中的生理变化和生长发育规律，对于揭示连作杨树人工林地力衰退机理具有重要意义。本研究以黑杨派欧美杨I-107 (Populus×euramericana ‘Neva’) 水培幼苗为试材，参照二代杨树人工林根际土壤中的酚酸含量（X），在改良Hoagland营养液中设置五个酚酸浓度梯度（CK、0.5X、1.0X、1.5X、2.0X），测定酚酸处理后第1、5、10、20、30和40天的根系生理生化特性和处理后第40天的根系形态数据。结果表明：低浓度酚酸（0.5X）可提高杨树根系超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性，而高浓度酚酸(1.0X、1.5X和2.0X)则显著抑制两种酶的活性（P＜0.05）。根系活力随酚酸浓度的提高而显著降低（P＜0.05）；根系丙二醛含量以及根系伤害度随酚酸浓度的提高而显著提高（P＜0.05）。酚酸能显著抑制杨树幼苗根系发育，平均根尖数、平均根长、平均根系表面积、平均根系分枝数以及平均根系分形维数显著降低，而幼苗根系直径则显著增大（P＜0.05）。酚酸影响下，根尖附近侧根发育受到抑制，根系形状逐渐由柱形向倒金字塔形和伞形过渡，高浓度酚酸导致根尖坏死。

英文摘要：
Effects of Exogenous Phenolic Acids on Root Physiologic Characteristics and Morphologic Development of Poplar Hydroponic Cuttings

Yang Yang1, Wang Huatian1, Wang Yanping1, Jiang Yuezhong2, Wang Zongqin1

1. Agro-Ecoenvironmental Laboratory of Shandong Agricultural University, Forestry College of Shandong Agricultural University, Taian Shandong 271018

2. Shandong Academy of Forestry, Jinan, Shandong, 250014

Abstract: The degradation of forestland productivity of continuous cropping poplar plantations was closely related to accumulation of phenolic acids in rhizospheric soil. The research on effects of phenolic acids on physiologic characteristics and morphologic developments of poplar roots were essential to reveal the degradation mechanism of poplar forestland productivity. Hydroponic cuttings of black poplar cultivar I-107 (Populus × euramericana ‘Neva’) were cultured in Hoagland nutrient solution added with phenolic acids of five levels(CK, 0.5X, 1.0X, 1.5X, 2.0X) according to actual content in soil of continuous poplar plantation. Physiologic indexes were studied respectively at the 1st d, 5th d, 10th d, 20th d, 30th d and 40th d after treated with phenolic acids, and data of root morphologic characteristics were obtained at the 40th d. The results showed that: Activity of superoxide dismutase(SOD) and peroxidase(POD) were both enhanced by low level phenolic acid(0.5X) and inhibited significantly by high level phenolic acids(1.0X, 1.5X, 2.0X)（P＜0.05）; root activity reduced significantly while the level of phenolic acids rose（P＜0.05）; root malonaldehyde(MDA) content and root injury degree were both significhantly enhanced by phenolic acids(P＜0.05). Number of root tips, mean root length, mean root area, mean root forks, mean root fractal dimension were all significantly inhibited by phenolic acids while mean root diameter was significantly promoted by phenolic acids(P＜0.05). Lateral roots near the tip of the main root were obvious inhibited by phenolic acids. While phenolic acids level rose, the general shape of root system was ranged from column aspect, inverted pyramid to umbrella aspect . Root tips necrotized under high level phenolic acid treatments.

Key words: Phenolic acids; poplar cuttings; allelopathy; root physiology; root morphology
